|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Minimum Order Quantity:||1G|
|Delivery Time:||3-10 DAY|
|Source:||E. Coli||Purified By:||HPLC|
|Format:||White Or White-like Or Yellowish Lyophilized||Specific Activity:||≥170 Units/mg Pro.|
|Contaminant Activity:||Less Than 10 Ppm Of Recombinant Trypsin.||Cas:||9025-24-5|
sequencing grade modified trypsin,
Stability of RCPB solution under -20℃ and repeated freezing-thawing
ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
Recombinant Carboxypeptidase B
1) Reagent A. 25 mM Tris-HCl Buffer with 100 mM Sodium Chloride, pH 7.65 at 25°C±0.5°C. (Prepare 100 ml in deionized water using Tris Hydrochloride, and Sodium Chloride. Adjust to pH 7.65 at 25°C with 1 M NaOH or 1M HCl.)
2) 1.0 mM Hippuryl-L-Arginine Solution (Hippuryl-L-Arg)
Weight 16.7 mg Hippuryl-L-Arginine (Sigma Prod. No. H-2508), dissolve in 50 ml Reagent A to obtain 1.0 mM (Hippuryl-L-Arg). PREPARE FRESH.).
3) RCPB Solution
Three batches RCPB lyophilized powder (RC110919, RC110106 and RC110304), prepare 10 mg/ml with reagent A, respectively, and separated into 30 tubes for each batch, stored under -20℃.
Long term stability of RCPB solution: The initial activity was measure just after preparation and taken as 100%. The activity was measure every 2 months, 3 parallel samples for each batch each time, take average, calculate residual rate of activity compared with initial one and then draw a stability curve.
Stability of RCPB solution during 10 cycles repeated freezing and thawing:The initial activity was measure just after preparation and taken as 100%. The activity of 3 samples of three batches were measured according SOP just after taking from -20℃ and thawing in room temperature. Repeated 10 times. Calculate residual rate of activity compared with initial one and then draw a stability curve.
RCPB solution (10mg/ml) is stable when kept under -20℃ in 1 year.
RCPB solution (10mg/ml) is stable during 10 cycles repeated freezing and thawing.
Fig 1. Long term stability of RCPB solution (10mg/ml) for 12 months kept under -20℃
Fig 2. Stability of RCPB solution (10mg/ml) during 10 cycles repeated freezing and thawing
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