|Place of Origin:||China, Shanghai|
|Certification:||NQA ISO 9001:2015|
|Model Number:||V8 Protease|
|Minimum Order Quantity:||50ug|
|Packaging Details:||100ug; ice packing, carton|
|Delivery Time:||5-8 work days|
|Supply Ability:||1,000,0000ug per month|
|Product Name:||Endoproteinase Glu-C||Species:||Staphylococcal Serine Proteinase|
|Band Name:||YAXINBIO||Specific Activity:||≥ 5.0 U/mg Pro.|
|Synonyms:||V8 Protease; Endoproteinase Glu-C; Endoproteinase Asp-C||Storage Temperature:||2-8°C|
glutamic acid protease,
CAS 66676-43-5 Sequencing Grade Staphylococcus Aureus V8 Protease
Sequencing Grade V8 Protease
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
UNIT DEFINITION: One unit is defined as the amount of enzyme required to catalytic substrate Z-Phe-Leu-Glu-4-nitranilide produced 1 μmol of 4-nitroaniline per minute at 25°C and pH 7.8 .
Approx.5U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37℃ with casein as the substrate).
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant V8 protease should be stored under 2-8°C in sealed container. Product is stable within 6 months after dissolved with1mM HCl and 50mM HAC at -20°C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C 12h. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
Contact Person: Miss Eland