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| Place of Origin: | China, Shanghai |
| Brand Name: | YAXINBIO |
| Certification: | NQA ISO 9001:2015 |
| Model Number: | SRCPB01 |
| Minimum Order Quantity: | 100ug |
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| Price: | $198/100ug |
| Packaging Details: | ice packaging, carton |
| Payment Terms: | T/T |
| Supply Ability: | 1000 per week |
| Product: | Sequencing Grade Carboxypeptidase B | Manufacturer: | YaxinBio |
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| Source: | E. Coli | Specific Activity: | NLT 200 Units/mg Pro |
| Contaminant Activity: | No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. | RELATED PRODUCT: | Sequencing Grade Chymotrypsin |
| Impact: | Produce Insulin | ||
| High Light: | trypsin endopeptidase,carboxypeptidase enzyme |
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Carboxypeptidase B, Proteomics Grade, For Producing Recombinant Insulin
Recombinant Carboxypeptidase B
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white, or yellowish powder |
| Specific activity | ≥170 units/mg pro. |
| Purity(SDS-PAGE) | Single major band |
| Molecular Weight(SDS-PAGE) | 33.8±3.4 kDa |
UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
RECOMMEND USAGE
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
Recommend Usage
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Contact Person: Miss Eland
Tel: +8613482039151
