Sequence analysis How to dissolve pure recombinant carboxypeptidase B, enzymatic digestion conditions, how to store?

1) How to dissolve the sequencing grade recombinant carboxypeptidase B lyophilized powder?

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.5-8.0.

2) What is the ratio of sequencing grade recombinant carboxypeptidase B and aimed protein?

The ratio to aimed protein is 1:50 to 1:100 (w/w).

3) What is the digestion buffer?

The recommended digestion buffer is: 25mM Tris-HCl pH7.0-9.0.

4) How to store the sequencing grade recombinant carboxypeptidase B solution?

Sequencing grade recombinant carboxypeptidase B lyophilized powder should be stored under 2℃-8℃ in a sealed container.

After dissolved, it should be stored under -20℃ or below. It is stable within 24 months.

5) What is the stability when sequencing grade recombinant carboxypeptidase B solution is repeated freezing and thawing?

No activity lose after 10 cycles repeated freezing and thawing.