How is the V8 protease dissolved and stored?

1) What is the usage optimum PH and buffer solution for ?

The recommend buffer is pH8.0-8.5 Tris-HCl or 50mM NH₄HCO₃ , and the digestion can be at 37 ℃.

2) What is the recommended storage condition?

V8 protease should be stored under -20℃ or below in sealed container.

Above 80% activity can be remained after 5 cycles repeated freezing -thawing.