How is the V8 protease dissolved and stored?
March 8, 2022
1) What is the usage optimum PH and buffer solution for ?
The recommend buffer is pH8.0-8.5 Tris-HCl or 50mM NH4HCO3 , and the digestion can be at 37 ℃.
2) What is the recommended storage condition?
V8 protease should be stored under -20℃ or below in sealed container.
Above 80% activity can be remained after 5 cycles repeated freezing -thawing.