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95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence

95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence

    • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence
    • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence
    • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence
    • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence
    • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence
  • 95% Purity Sequencing Grade Chymotrypsin , Amino Acid Sequence

    Product Details:

    Place of Origin: Shanghai China
    Brand Name: yaxin biotechnology
    Certification: NSF ISO 9001 2015
    Model Number: SRCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: $158
    Packaging Details: 100ug/1mg/10mg
    Delivery Time: 2-3 work days
    Supply Ability: 100000
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    Detailed Product Description
    Source: Recombinant E. Coli Appearance: White Or Off White Powder
    Specific Activity: ≥1500 USP Units/mg Pro. SDS-PAGE: Single Major Band
    SDS-PAGE (MW): 24.0±2.4 KDa Purity(HPLC): ≥ 95%

    Sequencing Grade Chymotrypsin whose amino acid sequence is identical to human chymotrypsin

    Sequencing Grade Chymotrypsin

    Catalog Number:SRCT10

    CAS: 9004-07-3
    EC: 3.4.21.1

    Storage temperature: 2-8℃

    Source: Sequencing grade chymotrypsin is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of sequencing grade chymotrypsin is identical to human chymotrypsin

     

    1. DESCRIPTION

    Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0.

     

    2. MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white powder
    Specific activity ≥1500 USP units/mg pro.
    SDS-PAGE Single major band
    SDS-PAGE (MW) 24.0±2.4 kDa
    Purity(HPLC) ≥ 95%

     

    3. RECOMMEND USAGE

    Prepare 1-10mg/ml sequencing grade chymotrypsin with 50mM HAC.When digest the protein, the target protein was dissolved with 50 mM or 100 mM NH4HCO3, and then add a certain amount of enzyme.The ratio to aimed protein is 1:50 to 1:1000 (w/w) at 30℃.When the temperauter is more than 30℃,enzyme will be self-cutting.

     

    4. STABILITY OF STORAGE AND TRANSPORT

    Stability of storage:Sequencing grade chymotrypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolving with 50mM HAc, store at 2-8 ℃,or use within 2 days.If the solution is stored at -20 ℃ or below, it is stable within 24 months. The solution should avoid repeated freezing and thawing and placing at room temperature a long time.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    5. PRODUCT ADVANTAGE

    Sequence grade recombinant protein: high specificity,good stability.

    Animal origin free: Sequencing grade chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

    Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

    Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

    Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    6. PRODUCT USAGE

    Sequencing grade chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

    7. RELATED PRODUCTS

    Recombinant enterokinase

    Sequencing grade trypsin

    Sequencing grade V8 protease

    Sequencing grade carboxypeptidase B

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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