|Place of Origin:||SHANGHAI CHINA|
|Certification:||NSF ISO 9001 2015|
|Model Number:||RCT10 1000u/mg pro|
|Minimum Order Quantity:||1mg|
|Delivery Time:||2-3 work days|
|Source:||Recombinant E. Coli||Appearance:||White Or Off White Powder|
|Specific Activity:||≥1000 USP Units/mg Pro.||SDS-PAGE:||Single Major Band|
|SDS-PAGE (MW):||24.0±2.4 KDa|
chymotrypsin enzyme,a genetically engineered protein which amino acid sequence is identical to human chymotrypsin
Recombinant Human Chymotrypsin
Storage temperature: 2-8℃
Source: Recombinant huamn chymotrypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human chymotrypsin is identical to human chymotrypsin
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥1000 USP units/mg pro.|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||24.0±2.4 kDa|
UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.
Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved wth 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
(1)Animal origin free: Recombinant human chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
(3)High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
(5)Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
(6)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
Used alone, or with other proteases to interact with digestive proteins, or other special uses such as organic synthesis.
Recombinant carboxypeptidase B
Sequencing grade chymotrypsin
Contact Person: Miss Eland