|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||1mg|
|Packaging Details:||1mg, 10mg, 100mg, 1g; ice packing, carton|
|Delivery Time:||2-3 work days|
|Source:||Recombinant E. Coli||Appearance:||White Or Off White Powder|
|Specific Activity:||≥2500 USP Units/mg Pro.||Protein Content:||≥ 90%|
|Purity(SDS-PAGE):||Single Major Band||Molecular Weight(SDS-PAGE):||24.0±2.4 KDa|
stem cell culture,
Recombinant Human Trypsin, White or Off-white Powder, CAS 9002-07-7
Recombinant Human Trypsin
Storage temperature: 2-8℃
Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥2500 USP units/mg pro.|
|Protein Content||≥ 90%|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
(1)Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
(3)High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
(5)Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
(6)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.
Recombinant carboxypeptidase B
Sequencing grade trypsin
Contact Person: Miss Eland