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NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine

NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine

    • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine
    • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine
    • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine
    • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine
    • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine
  • NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine

    Product Details:

    Place of Origin: CHINA
    Brand Name: Ya Xin
    Certification: NSF ISO 9001 2015
    Model Number: Recombinant Carboxypeptidase B

    Payment & Shipping Terms:

    Minimum Order Quantity: 1mg
    Price: $67/mg
    Packaging Details: 1mg,10mg,100mg
    Delivery Time: 2-3 work days
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    Detailed Product Description
    SOURCE: Recombinant E. Coli Appearance: White Or Off White, Or Yellowish Powder
    Specific Activity: ≥170 Units/mg Pro. Purity(SDS-PAGE): Single Major Band
    Molecular Weight(SDS-PAGE): 33.8±3.4 KDa Cas: 9025-24-5
    Source: Recombinant Carboxypeptidase B Is A Genetically Engineered Protein Expressed In E.coli

    Recombinant Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine

    NSF Recombinant Carboxypeptidase B Catalyzes Hydrolysis Of The Basic Amino Acids Lysine , Arginine And Histidine

    Recombinant Carboxypeptidase B

    Catalog Number:RCPB01

    CAS: 9025-24-5
    EC: 3.4.17.2

    Storage temperature: 2-8℃

    Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

     

    1.DESCRIPTION

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

     

    2.MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white, or yellowish powder
    Specific activity ≥170 units/mg pro.
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 33.8±3.4 kDa

    UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    3.RECOMMEND USAGE

    Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

     

    4.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    5.PRODUCT ADVANTAGE

    (1)Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

    (2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    (3)High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.

    (4)Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

    (5)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    6.PRODUCT USAGE

    The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds.

     

    7.RELATED PRODUCTS

    Recombinant trypsin

    Sequencing grade trypsin

    Recombinant human trypsin

    Recombinant enterokinase

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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