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ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

    • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
    • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
    • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
    • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
    • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u
  • ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

    Product Details:

    Place of Origin: CHINA
    Brand Name: yaxin
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 1G
    Price: $1950
    Packaging Details: 1G,10G,50G
    Delivery Time: 3-10 DAY
    Payment Terms: T/T
    Supply Ability: 1000G
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    Detailed Product Description
    Source: E. Coli Purified By: HPLC
    Format: White Or White-like Or Yellowish Lyophilized Specific Activity: ≥170 Units/mg Pro.
    Contaminant Activity: Less Than 10 Ppm Of Recombinant Trypsin. Cas: 9025-24-5

    Stability of RCPB solution under -20 and repeated freezing-thawing

     

    ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

     

    Recombinant Carboxypeptidase B

     

    REAGENT

     

    1) Reagent A. 25 mM Tris-HCl Buffer with 100 mM Sodium Chloride, pH 7.65 at 25°C±0.5°C. (Prepare 100 ml in deionized water using Tris Hydrochloride, and Sodium Chloride. Adjust to pH 7.65 at 25°C with 1 M NaOH or 1M HCl.)

    2) 1.0 mM Hippuryl-L-Arginine Solution (Hippuryl-L-Arg)

    Weight 16.7 mg Hippuryl-L-Arginine (Sigma Prod. No. H-2508), dissolve in 50 ml Reagent A to obtain 1.0 mM (Hippuryl-L-Arg). PREPARE FRESH.).

    3) RCPB Solution

    Three batches RCPB lyophilized powder (RC110919, RC110106 and RC110304), prepare 10 mg/ml with reagent A, respectively, and separated into 30 tubes for each batch, stored under -20℃.

     

     

    METHOD:

     

    Long term stability of RCPB solution: The initial activity was measure just after preparation and taken as 100%. The activity was measure every 2 months, 3 parallel samples for each batch each time, take average, calculate residual rate of activity compared with initial one and then draw a stability curve.

    Stability of RCPB solution during 10 cycles repeated freezing and thawing:The initial activity was measure just after preparation and taken as 100%. The activity of 3 samples of three batches were measured according SOP just after taking from -20℃ and thawing in room temperature. Repeated 10 times. Calculate residual rate of activity compared with initial one and then draw a stability curve.

     

     

    CONCLUSION:

     

    RCPB solution (10mg/ml) is stable when kept under -20℃ in 1 year.

    RCPB solution (10mg/ml) is stable during 10 cycles repeated freezing and thawing.

     

    ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

    Fig 1. Long term stability of RCPB solution (10mg/ml) for 12 months kept under -20℃

     

    ISO Recombinant Carboxypeptidase B Solution Under -20℃ And Repeated Freezing-Thawing , Specific Activity ≥170 u

    Fig 2. Stability of RCPB solution (10mg/ml) during 10 cycles repeated freezing and thawing

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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