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High Purity, Recombinant Kex2 Protease, Purified by HPLC

High Purity, Recombinant Kex2 Protease, Purified by HPLC

  • High Purity, Recombinant Kex2 Protease, Purified by HPLC
  • High Purity, Recombinant Kex2 Protease, Purified by HPLC
  • High Purity, Recombinant Kex2 Protease, Purified by HPLC
  • High Purity, Recombinant Kex2 Protease, Purified by HPLC
High Purity, Recombinant Kex2 Protease, Purified by HPLC
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: Re15
Payment & Shipping Terms:
Minimum Order Quantity: 10U
Price: 545USD/mg
Packaging Details: ice packaging, carton
Payment Terms: T/T
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Detailed Product Description
Brand: YaxinBio Source: Pichia Pastoris
Appearance: White Or Off White Powder Specific Activity: ≥10 Units/mg Pro.
Purity(SDS-PAGE): Single Major Band Molecular Weight(SDS-PAGE): 67 ± 6.7 KDa
High Light:

Recombinant protease

,

saccharomyces cerevisiae yeast

High Purity, Recombinant Kex2 Protease, Purified by HPLC

 

Recombinant Kex2 Protease

 

Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease;YSCF Protenase

Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris

Storage temperature: 2-8℃

Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2

 

Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.

 

 

Purified by HPLC
Format Liquid in10mM NaAc-HAc(PH 5.2) and 2mM Ca2+
Specific activity ≥10u/mg
12.5%SDS-PAGE Single main stripe
Mol.weight 67±6.7 kDa

 

Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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