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High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography

High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography

    • High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography
    • High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography
    • High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography
    • High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography
  • High Purity Recombinant Kex2 Protease and purified by high pressure liquid chromatography

    Product Details:

    Place of Origin: CHINA
    Brand Name: YAXINBIO
    Certification: ISO 9001 2015
    Model Number: RE15

    Payment & Shipping Terms:

    Minimum Order Quantity: 10U
    Price: USD3850
    Packaging Details: 0.5U、10U、50U
    Payment Terms: T/T
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    Detailed Product Description
    Brand: Yaxinbio Source: Pichia Pastoris
    Appearance: White Or Off White Powder Specific Activity: ≥5 Units/mg Pro.
    Purity(SDS-PAGE): Single Major Band Molecular Weight(SDS-PAGE): 67 ± 6.7 KDa

    High Purity,Recombinant Kex2 Protease and purified by high pressure liquid chromatography

     

    Recombinant Kex2 Protease

     

    Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease;YSCF Protenase

    Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris

    Storage temperature: 2-8℃

    Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2

     

    Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.

     

     

    Purified by HPLC
    Format Liquid in10mM NaAc-HAc(PH 5.2) and 2mM Ca2+
    Specific activity ≥5u/mg
    12.5%SDS-PAGE Single main stripe
    Mol.weight 67±6.7 kDa

     

    Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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