|Place of Origin:||china|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||1mg|
|Delivery Time:||8 days|
|Catalog Number:||Re15||Source:||Pichia Pastoris|
|Product:||Recombinant Kex2 Protease||Packaging:||50µg/each|
|Transport Temperature:||≤ 8 °C.||Specific Activity:||≥5u/mg|
|Purity:||Single Main Stripe||Express:||Pichia Pastoris|
Recombinant Kex2 Protease Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
Recombinant Kex2 Protease
Source:Saccharomyces cerevisiae Kex2, expressed in Pichia pastoris
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
|Format||Liquid in10mM NaAc-HAc(PH 5.2) and 2mM Ca2+|
|12%SDS-PAGE||Single main stripe|
Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.
Recommended storage temperature: -20 °C or below. It is stable after 5 cycles freezing and thawing.
Transport temperature: ≤ 8 °C.
It should be stored in 10mM NaAc-HAc(PH 5.2) and 2mM Ca2+.
|Recombinant Kex2 Protease||≥5u/mg||50µg/each||YaxinBio|
Contact Person: Miss Eland