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Recombinant Kex2 Protease Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris

Recombinant Kex2 Protease Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris

    • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
    • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
    • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
    • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
    • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris
  • Recombinant Kex2 Protease  Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris

    Product Details:

    Place of Origin: china
    Brand Name: YAXINBIO
    Certification: ISO 9001 2015
    Model Number: Re15

    Payment & Shipping Terms:

    Minimum Order Quantity: 1mg
    Price: $3850
    Packaging Details: 50ug
    Delivery Time: 8 days
    Payment Terms: T/T
    Supply Ability: 10000g
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    Detailed Product Description
    Catalog Number: Re15 Source: Pichia Pastoris
    Product: Recombinant Kex2 Protease Packaging: 50µg/each
    Transport Temperature: ≤ 8 °C. Specific Activity: ≥5u/mg
    Purity: Single Main Stripe Express: Pichia Pastoris

    Recombinant Kex2 Protease Single Main Stripe 12.5% SDS - PAGE expressed in Pichia pastoris

     

    Recombinant Kex2 Protease 

     

     

    Catalog Number:Re15

    EC:3.4.21.61

    Source:Saccharomyces cerevisiae Kex2, expressed in Pichia pastoris

     

     

    Description

     

    Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.

     

     

    Main Features

     

    Source Pichia pastoris
    Purified by HPLC
    Format Liquid in10mM NaAc-HAc(PH 5.2) and 2mM Ca2+
    Specific activity ≥5u/mg
    12%SDS-PAGE Single main stripe
    Mol.weight 67±6.7 kDa

     

    Unit Definition:One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA as the substrate.

     

     

    Storage Instructions

     

    Recommended storage temperature: -20 °C or below. It is stable after 5 cycles freezing and thawing.

    Transport temperature: ≤ 8 °C.

    It should be stored in 10mM NaAc-HAc(PH 5.2) and 2mM Ca2+.

     

     

    Product Infomation

     

    Product Specific activity Packaging Manufacturer
    Recombinant Kex2 Protease ≥5u/mg 50µg/each YaxinBio

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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