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Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature

Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature

    • Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0  With 2-8°C Storage Temperature
    • Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0  With 2-8°C Storage Temperature
    • Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0  With 2-8°C Storage Temperature
    • Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0  With 2-8°C Storage Temperature
  • Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0  With 2-8°C Storage Temperature

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: Re15

    Payment & Shipping Terms:

    Minimum Order Quantity: 50ug
    Price: USD142
    Packaging Details: 50ug
    Delivery Time: 4days
    Payment Terms: T/T
    Supply Ability: 10000G
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    Detailed Product Description
    Activity: ≥5u/mg Purification: HPLC
    Character: Freeze-dried Powder Molecular: 67±6.7 KDa
    Perfect PH: 9.0 Nature: Double Base Enzyme
    Temperature: 2-8℃

    Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature

     

    Kex-2 Protease

     

    PRODUCT INFORMATION
    Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids.At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

     

    MAIN FEATURES

    Source Pichia pastoris
    Appearance White or off white powder
    Specific activity ≥5.0 units/mg pro.
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 67±6.7 kDa

    UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.

     

    RECOMMEND USAGE

    Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca2 + or HEPES, 5 mM Ca2 +. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2 + buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 + or HEPES, 5 mM Ca2 + buffer.

    Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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