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Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells

Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells

    • Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells
    • Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells
    • Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells
    • Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells
  • Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RTS04

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ml
    Price: consult
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 10000L per week
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    Detailed Product Description
    Product: Recombinant Trypsin Solution Cat. No.: RTS04
    Specific Activity: 2000 BAEE U/ml Packaging: 100ml,500 Ml,bulk
    Convenient: Dissociate Adherent Cells RELATED PRODUCT: Recombinant (Porcine/Human )Trypsin

    Trypsin Solution for Cell Separation Recombinant Trypsin Solution

     

     

    Description

     

    Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin  expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

     

    Features And Benefits

     

    Recombinant trypsin

    Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin

    inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.

     

    Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

    Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform

    to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

     

    Advanges

     

    High safety The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
    High purity

    Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

    Good work without EDTA No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
    Convenient

    Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

     

     

     

     

     

     

     

     

     

     

     

    Unit Definition:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in

    a reaction volume of 3.2ml at pH7.6 and 25℃ (1cm light path). 1 USP unit = 3 BAEE unit

     

    Instructions for use

     

    The following instructions are applicable for most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines (see notes below).

    1. Wash the cells with DPBS w/o Ca, Mg (Cat No. 02-023-1).

    2. Add 1ml of Recombinant Trypsin Solution to each T-25 tissue culture flask.

    3. Incubate the flask at 37°C for 2 min or longer as necessary. Verify cell’s detachment using inverted microscope.

    4. When cells are completely detached, add 5-10 ml of culture medium (MSC NutriStem® XF, Cat. No. 05-200-1). Alternatively use diluted (1:50, in DPBS) Soybean Trypsin Inhibitor (SBTI, Cat. No. 03-048-1).

    5. Pellet the cells by centrifugation, and discard the supernatant.

    6. Resuspend the cells in growth medium, and seed as desired.

     

    Recombinant Trypsin For Cell Separation Recombinant Dissociate Adherent Cells

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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