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Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro

Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro

    • Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro
    • Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro
    • Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro
    • Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro
  • Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 1mg
    Price: 385$/mg
    Packaging Details: dry ice
    Delivery Time: five days
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Carboxypeptidase B EC: 3.4.17.2
    CAS: 9025-24-5 Specific Activity: NLT 200 Units/mg Pro
    Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Trypsin (sequencing Grade)

    Recombinant Carboxypeptidase B Proteomics Grade Enzyme with purity 200 u / mg Pro

     

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The

    enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

    Application

     

    Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine

    and histidine from the C-terminal end of polypeptides.

    Antibody quality control.

     

    Main Features

     

    Purity/Purification Greater than 90% as determined by SDS-PAGE.
    Form/Format The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
    Sterile Filtered lyophilized powder.
    Unit Defenition One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 degree C, pH-7.65.
    Solubility It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
    Biological Activity 200 units/mg protein.
    Preparation and Storage Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
    Other Notes for Cpb1 recombinant protein Small volumes of Cpb1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
    Searchable Terms for Cpb1 purchase anti-Cpb1 cost; Buy Cpb1 recombinant protein, Carboxypeptidase-B Recombinant Protein (MBS143539) product datasheet at MyBioSource, Recombinant Proteins; ENZYMES; Enzymes; Peptidase; 24271; CBPB1_RAT; Cpb1 recombinant protein price; recombinant protein for Cpb1

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

    Recommend Usage

     

    Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratioto aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

     

    Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u / mg Pro

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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