|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||1mg|
|Packaging Details:||1mg, 10mg, 100mg, 1g; dry ice, carton|
|Delivery Time:||five days|
|Supply Ability:||1000g per week|
|Product:||Sequencing Grade Carboxypeptidase B||EC:||188.8.131.52|
|CAS:||9025-24-5||Specific Activity:||NLT 200 Units/mg Pro|
|Contaminant Activity:||No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant.||RELATED PRODUCT:||Recombinant Trypsin (sequencing Grade)|
Recombinant Carboxypeptidase B, Proteomics Grade, Enzyme purity 200U/mg Pro.
Recombinant Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The
enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine
and histidine from the C-terminal end of polypeptides.
Antibody quality control.
|Purity/Purification||Greater than 90% as determined by SDS-PAGE.|
|Form/Format||The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Sterile Filtered lyophilized powder.
|Unit Defenition||One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 degree C, pH-7.65.|
|Solubility||It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.|
|Biological Activity||200 units/mg protein.|
|Preparation and Storage||Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.|
|Other Notes for Cpb1 recombinant protein||Small volumes of Cpb1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.|
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Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratioto aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
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