|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Delivery Time:||6 days|
|Supply Ability:||1000g per week|
|Specific Activity:||≥170 U/mg Pro||Purity:||> 95%|
Recombinant Enzyme Carboxypeptidase B for Mass Spectrometry with 170u / mg pro
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 33.8kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered lyophilized powder.
Formulation: The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Solubility: It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of
contamination and decreases the chances of activity loss in the process of transport and storage.
1) Recombinant carboxypeptidase B provides increased specific activity and eliminates
contaminating proteases activities found in extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3)Less than 10ppm of recombinant trypsin.
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3,
eliminate the risk of virus presence, or of any other potential adventitious agents found in
animal-derived carboxypeptitase B
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The
ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in
sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃,
It is stable within 24 months and above 90% activity remained after 10 times repeated freezing
Contact Person: Miss Eland