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ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein

ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein

    • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein
    • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein
    • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein
    • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein
  • ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 98$/100ug
    Packaging Details: ice packaging
    Delivery Time: 7 weekdays
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Cat. No.: SRT0201
    CAS: 9002-07-7 Specific Activity: >4500 USP Units/mg Pro
    EC: 3.4.21.4 Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.

    ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein

     

    Sequencing Grade Trypsin Endoproteinase Lys-c for Digesting Specific Protein

     

     

    Sequencing Grade Trypsin

     

    DESCRIPTION

    YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white, or yellowish powder
    Specific activity ≥4500 USP units/mg pro.
    SDS-PAGE Single major band
    Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
    Purity(HPLC) ≥95%

     

    Unit Definition

    One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    Application

    Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

    Reconmend Usage

    1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

    2. When using this product, thaw it at room temperature, and mix gently before use.

    3. No activity lost when freeze-thaw 5 cycles.

     

    Storage Instructions

    1. The solution should be stored under -70℃, It is stable within 24 months.

    2. Above 95% activity remained after 5 times repeated freezing and thawing.

    3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

     

    PRODUCT USAGE

    The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

     

    ISO 9001 Sequencing Grade Trypsin Endoproteinase Lys - c for Digesting Specific Protein

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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