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Animal origin free trypsin E.coli recombinant Trypsin 3800 u/mg pro

Animal origin free trypsin E.coli recombinant Trypsin 3800 u/mg pro

    • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro
    • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro
    • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro
    • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro
    • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro
  • Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RPT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 1g
    Price: 450$/g
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Purified By: HPLC Format: White Lyophilized Powder
    Specific Activity: ≥3800 USP U/mg Pro Purity(RP-HPLC): NLT 70% β-trysin, NMT 20% α-trypsin
    Source: E. Coli

    Animal Origin Free Trypsin E.coli Recombinant Trypsin 3800 u/mg pro

     

    Recombinant Trypsin

     

    Description

     

    Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

    by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

    Calculation

    BAEE Units/ml enzyme = (ΔA253nm/min Test - ΔA253nm/min Blank)(df)
    (0.001) (0.20)

     

     

     

    df = Dilution factor
    0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction

    mix 0.20 = Volume (in milliliters) of enzyme used

    Units/mg solid= units/ml enzyme
    mg solid/ml enzyme

     

     

     

    Notes
    1.This assay procedure is not to be used to assay immobilized trypsins
    2.For the USP/NF procedure refer to the USP monograph
    3.This procedure is for informational purposes. For a current copy of Sigma’s quality control procedure contact our Technical Service Department.

    Unit Conversions
    1 TAME µM Unit = 0.27 BAEE µM Units
    1 BAEE µM Unit = 3.64 TAME Units
    1 TAME µM Unit = 55 BAEE A253 Units
    1 BAEE A253 Unit = 0.018 TAME µM Unit
    1 TAME µM Unit = 180 TAME A247 Units
    1 TAME A247 Unit = 0.33 BAEE Units
    1 USP Unit = 3.0 BAEE Units
    1 NF Unit = 1.1 USP Units

     

    Advantages

     

    Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

    and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

    Porcine Trypsin belongs to the AOF level 3.

    Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

     

    Recommend Usage

     

    Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

     

    Storage Instructions

     

    Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

    within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

    above 90% activity remained after 10 times repeated freezing and thawing.

    Animal origin free trypsin E.coli recombinant Trypsin   3800 u/mg pro

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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