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Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

    • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease
    • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease
    • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease
    • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease
    • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease
  • Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RPT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 65$/10mg
    Packaging Details: ice packaging
    Delivery Time: 7 days
    Payment Terms: T/T
    Supply Ability: 100000g per week
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    Detailed Product Description
    Product: Recombinant Porcine Trypsin Cat. No.: RPT0201
    CAS: 9002-07-7 EC: 3.4.21.4
    Related Product: Recombinant Carboxypeptidase B White Lyophilized: HPLC
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    porcine trypsin

    ,

    trypsin enzyme

    Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

     

    Recombinant Trypsin

    Description

     

    Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The  enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,

    e.g.EDTA.Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli  and purified by high pressure liquid chromatography. There are no contaminating enzyme  activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF  are contained in the preparation.

    Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site.

    Trypsin will hydrolyze ester and amide linkages of several synthetic substrates.

     

    Advantages

     

    Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

    Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant  Porcine Trypsin belongs to the AOF level 3.

    High purity:

    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

     

    Storage Instructions

     

    Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable  within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months  and above 90% activity remained after 10 times repeated freezing and thawing.

     

    Main Features

     

    Source

    E. Coli

    Format

    White lyophilized

    Specific activity

    ≥3800 USP units/mg pro

    Purity(RP-HPLC)

    NLT 70% β-trysin, NMT 20% α-trypsin

    Contaminant activity

    No chymotrypsin, carboxypeptidase A, and other protease contaminant.

     

     

     

     

     

     

     

    Unit Dediniton

     

    One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    Recommend Usage

     

    Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is 7-10.

     

    Serine Enzyme Recombinant Trypsin Lysine Protease or Arginine Protease

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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