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Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation

Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation

    • Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation
    • Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation
    • Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation
    • Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation
  • Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RTS04

    Payment & Shipping Terms:

    Minimum Order Quantity: 25ml
    Price: consult
    Packaging Details: ice packaging
    Delivery Time: Six days
    Payment Terms: T/T
    Supply Ability: 1000L per weel
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    Detailed Product Description
    Product: Recombinant Trypsin Solution Purity: High Purity
    Specific Activity: 2000 BAEE U/ml Packaging: 25ml,100 Ml,bulk
    Convenient: Dissociate Adherent Cells RELATED PRODUCT: Recombinant Porcine Trypsin

    Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation 

     

    Recombinant trypsin solution(RTS)

    Description

     

    Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin  expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

     

    Method Of Use

     

    Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.

    Step 2. Dissolve the trypsin powder.

    Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.

     

    Notes

     

    Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.

    Note 2. If necessary, after dissolving, immediately dispense into suitable ; containers, then stored under -20℃. To use it, dissolve it at room temperature. 

    Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.  

     

    Instructions for use

     

    The following instructions are applicable for most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines (see notes below).

    1. Wash the cells with DPBS w/o Ca, Mg (Cat No. 02-023-1).

    2. Add 1ml of Recombinant Trypsin Solution to each T-25 tissue culture flask.

    3. Incubate the flask at 37°C for 2 min or longer as necessary. Verify cell’s detachment using inverted microscope.

    4. Pellet the cells by centrifugation, and discard the supernatant. 6. Resuspend the cells in growth medium, and seed as desired.

    Trypsin Solution 1 / 2500 Animal Components Free Trypsin for Cell Dissociation

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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