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Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

    • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
    • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
    • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
    • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
    • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RSPA05

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 79$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Brand: Yaxinbio Substrate: Porous Polystyrene-divinylbenzene
    Detergents: Alkaline CIP Solvents Storage: 20% Ethanol 2-8 ℃
    PH: PH 2-10 Temperature Range: 2-8℃

    Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

     

     Recombinant protein A                   

     

    Application

     

    The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

    Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

     

    Product Specification

     

    Test/Method                    

    Specification

    Bioburden

    No organisms detected.

    12% SDS-PAGE

    Single major band

    Coomassie Stain

    25kD

    UV Spectrum

    E275/250 ≥1.2,

    E0.1% at 275nm=0.17-0.23 

    UV spectrometry

    No DNA or RNA detected

    Triton Content

    No Triton contaminant.

    Format

    Lyophilized

    pH stable range

    pH 1-13

     

     

     

     

     

     

     

     

     

     

     

     

     

    PRODUCT ADVANTAGE

    • Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
    • SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
    • The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
    • Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
    • High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
    • Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
    • Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
    • Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    Storage Instructions

    Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

     

    Usage

    For Research or Manufacturing Purpose Only. Not for Human.

    Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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