|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||10mg, 100mg, 1g; ice packaging, carton|
|Supply Ability:||1000g per week|
|Detergents:||Alkaline CIP Solvents||Storage:||20% Ethanol 2-8 ℃|
|PH:||PH 2-10||Temperature Range:||2-8℃|
SPA protein A,
Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
Recombinant protein A
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|
Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.
For Research or Manufacturing Purpose Only. Not for Human.
Contact Person: Miss Eland