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Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

    • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A
    • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A
    • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A
    • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A
    • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A
  • Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RSPA05

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 79$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Brand: Yaxinbio Substrate: Porous Polystyrene-divinylbenzene
    Ligand: Recombinant Protein A Recommend Flow Rate: 300-700 Cm/h
    DBC: At Least 300 Mg IgG/mL Media At 500 Cm/h Maximum Pressure: 1500 PSI

    Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

     

     Protein A

    CAS:91932-65-9

    Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.

                        

    Application(1)

     

    The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

    Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

     

    APPLICATION(2)

     

    The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

     

    MAIN FEATURES

    Appearance White or off white powder
    Solubility Clear liquid
    Bioburden ≤ 1 cfu/ mg
    SDS-PAGE Single major band
    SDS-PAGE (MW) 25000±1000 Daltons
    UV Spectrum E275/250 ≥ 1.2
    E0.1% at 275nm = 0.17-0.23
    Purity (HPLC) ≥ 95%

     

    STABILITY OF STORAGE AND TRANSPORT

     

    Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

    After dissolved with pure water, it should be stored under -20℃.

     

    Stability of transport: The product is stable by blue ice insulation transport.

     

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    Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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