|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||100ug|
|Packaging Details:||ice packaging|
|Supply Ability:||1000g per week|
|EC:||126.96.36.199||Purity:||NLT 95% By HPLC Analysis.|
|Product:||Recombinant Carboxypeptidase B||Source:||E. Coli|
|Physical Form:||Lyophilized Powder||RELATED PRODUCT:||Recombinant Enterokinase|
Chymotrypsin Proteomics Grade Chymotrypsin For Peptide Mass Fingerprinting
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥1500 USP units/mg pro.|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||24.0±2.4 kDa|
Prepare 1-10mg/ml sequencing grade chymotrypsin with 50mM HAC.When digest the protein, the target protein was dissolved with 50 mM or 100 mM NH4HCO3, and then add a certain amount of enzyme.The ratio to aimed protein is 1:50 to 1:1000 (w/w) at 30℃.When the temperature is more than 30℃,enzyme will be self-cutting.
1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
Contact Person: Miss Eland