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Chymotrypsin Proteomics Grade Chymotrypsin For Peptide Mass Fingerprinting

Chymotrypsin Proteomics Grade Chymotrypsin For Peptide Mass Fingerprinting

    • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting
    • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting
    • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting
    • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting
    • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting
  • Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 158$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    EC: 3.4.21.1 Purity: NLT 95% By HPLC Analysis.
    Product: Recombinant Carboxypeptidase B Source: E. Coli
    Physical Form: Lyophilized Powder RELATED PRODUCT: Recombinant Enterokinase

    Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting

     

     

    Description

     

    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

    The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

     

    MAIN FEATURES

     

    Source Recombinant E. coli
    Appearance White or off white powder
    Specific activity ≥1500 USP units/mg pro.
    SDS-PAGE Single major band
    SDS-PAGE (MW) 24.0±2.4 kDa
    Purity(HPLC) ≥ 95%

     

     

    RECOMMEND USAGE

     

    Prepare 1-10mg/ml sequencing grade chymotrypsin with 50mM HAC.When digest the protein, the target protein was dissolved with 50 mM or 100 mM NH4HCO3, and then add a certain amount of enzyme.The ratio to aimed protein is 1:50 to 1:1000 (w/w) at 30℃.When the temperature is more than 30℃,enzyme will be self-cutting.

     

     

    Application

     

    1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

     

     

    Chymotrypsin Proteomics Grade   Chymotrypsin For Peptide Mass Fingerprinting

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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