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Chymotrypsin Proteomics Grade enzymes For Digestion Reactions In-Solution

Chymotrypsin Proteomics Grade enzymes For Digestion Reactions In-Solution

    • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution
    • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution
    • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution
    • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution
    • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution
  • Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 158$/100ug
    Packaging Details: ice packaging;
    Payment Terms: T/T
    Supply Ability: 1000 per week
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    Detailed Product Description
    EC: 3.4.21.1 SOURCE: Expressed In E. Coli
    Physical Form: Lyophilized Powder Specific Activity: NLT 1500 USP Units/mg Pro
    Contaminant Activity: No Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Chymotrypsin

    Chymotrypsin Proteomics Grade  Chymotrypsin For Digestion Reactions In-Solution

     

    Sequencing grade human chymotrypsin 

    Description

     

    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

    The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

    Recommend Usage

     

    For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.

     

    Storage Instructions

     

    Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Chymotrypsin Proteomics Grade  enzymes For Digestion Reactions In-Solution

     

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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