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Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis

Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis

    • Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis
    • Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis
    • Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis
    • Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis
  • Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: SRCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 158$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    EC: 3.4.21.1 Purity: NLT 95% By HPLC Analysis.
    Physical Form: Lyophilized Powder Specific Activity: NLT 1500 USP Units/mg Pro
    Contaminant Activity: No Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Sequencing Grade Trypsin
    Brand: Yaxin

    Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis

     

    Sequence grade Chymotrypsin 

    Description

     

    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

    The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

     

    Application

     

    1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

     

    Recommend Usage

     

    For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.

     

    Storage Instructions

     

    Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     

    Chymotrypsin Enzyme Sequence Grade for Protein Identification Peptide Fingerprinting Analysis

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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