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Sequencing Grade Modified Trypsin Used For Protein Identification

Sequencing Grade Modified Trypsin Used For Protein Identification

    • Sequencing Grade Modified Trypsin Used For Protein Identification
    • Sequencing Grade Modified Trypsin Used For Protein Identification
    • Sequencing Grade Modified Trypsin Used For Protein Identification
    • Sequencing Grade Modified Trypsin Used For Protein Identification
    • Sequencing Grade Modified Trypsin Used For Protein Identification
  • Sequencing Grade Modified Trypsin Used For Protein Identification

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Manufacturer: YaxinBio
    Purified By: HPLC Specific Activity: NLT 4500 USP U/mg Pro
    Purity(HPLC): ≥95% SDS-PAGE: Single Major Band

    Sequencing Grade Modified Trypsin Used For Protein Identification

     

    Sequencing Grade Trypsin

    Storage temperature: 2-8℃

    Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli

    Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin

     

    DESCRIPTION

    YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    RECOMMEND USAGE

    It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

     

    STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

    It is no activity loss after 5 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

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    Sequencing Grade Modified Trypsin Used For Protein Identification

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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