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Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade

Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade

    • Animal Components Free Carboxypeptidase B For Arg-C And lys-C   Sequencing Grade
    • Animal Components Free Carboxypeptidase B For Arg-C And lys-C   Sequencing Grade
    • Animal Components Free Carboxypeptidase B For Arg-C And lys-C   Sequencing Grade
    • Animal Components Free Carboxypeptidase B For Arg-C And lys-C   Sequencing Grade
  • Animal Components Free Carboxypeptidase B For Arg-C And lys-C   Sequencing Grade

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
    Source: E. Coli Specific Activity: NLT 200 Units/mg Pro
    Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Sequencing Grade Chymotrypsin

    Recombinant Carboxypeptidase B

     

    Catalog Number:RCPB01

    CAS: 9025-24-5
    EC: 3.4.17.2

    Storage temperature: 2-8℃

    Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

     

    1.DESCRIPTION

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

     

    2.MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white, or yellowish powder
    Specific activity ≥170 units/mg pro.
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 33.8±3.4 kDa

    UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    3.RECOMMEND USAGE

    Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

     

    4.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.

    It is no activity loss after 10 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    5.PRODUCT ADVANTAGE

    ① Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not

    used in the production process.

    ② Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and

    the product quality is stable.

    ③ High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host

    protein residues is less than the limits of biological products.

    ④ Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

     

    ⑤ Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

    requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

    ⑥ Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    6.PRODUCT USAGE

    The production of recombinant insulin and its analogues;

    the quality test of recombinant antibodies;

    the determination of C-terminal amino acids of proteins;

    the production of other recombinant polypeptides;

    and the enzymatic synthesis of certain specific compounds.

     

    7.RELATED PRODUCTS

    Recombinant trypsin

    Sequencing grade trypsin

    Recombinant human trypsin

    Recombinant enterokinase

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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