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Recombinant Carboxypeptidase B expressed in E.coli is impact for Proteomics Grade

Recombinant Carboxypeptidase B expressed in E.coli is impact for Proteomics Grade

    • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade
    • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade
    • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade
    • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade
    • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade
  • Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
    Source: E. Coli Purified By: HPLC
    Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Human Trypsin
    Source: Expressed In E.coli Impact: Proteomics Grade

    Recombinant Carboxypeptidase B expressed in E.coli is impact for Proteomics Grade

     

    Recombinant Carboxypeptidase B

     

    Description

     

    Carboxypeptidase B is a metalloenzyme, which is widely used for commercial and research purposes. Commercially available CPB purified from porcine or bovine pancreas is very expensive, and is not totally free from other proteases. In order to express the rat proCPB in Pichia pastoris, total RNA extracted from SD rat pancreas cells was reversely transcripted to synthesize cDNA, and the proCPB ORF was synthesized by PCR. After digestion with Xho I and EcoR I , the fragment was inserted into pPIC9, and the recombinant plasmid was named as pPIC9-proCPB. By digestion with Sac I , the lined pPIC9-proCPB was transformed into Pichia pastoris strains GS115 with PEG1000 and integrated into their genomes. In the inducement of methanol, recombinant proCPB was successfully expressed in Pichia pastoris, and could be secreted into the supernatant in the culture.

     

    Main Features

     

    Purity by HPLC

    ≥ 95%

    Format

    Lyophilized

    Specific activity

    ≥ 200 units/mg pro

    Contaminant activity

    No chymotrypsin, carboxypeptidase A, or other proteases contaminant.

     

     

     

     

     

     

     

    Application

     

    Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

    Antibody quality control.

     

    Recommend Usage

     

    Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to

    aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable

    within 24 months and no activity lose after 10 times repeated freezing and thawing.

    Recombinant Carboxypeptidase B expressed in E.coli is impact  for Proteomics Grade

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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