|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||100ug|
|Packaging Details:||ice packaging|
|Supply Ability:||1000g per week|
|Product:||Sequencing Grade Carboxypeptidase B||Manufacturer:||YaxinBio|
|Source:||E. Coli||Purified By:||HPLC|
|Contaminant Activity:||No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant.||RELATED PRODUCT:||Recombinant Human Trypsin|
|Source:||Expressed In E.coli||Impact:||Proteomics Grade|
Recombinant Carboxypeptidase B expressed in E.coli is impact for Proteomics Grade
Recombinant Carboxypeptidase B
Carboxypeptidase B is a metalloenzyme, which is widely used for commercial and research purposes. Commercially available CPB purified from porcine or bovine pancreas is very expensive, and is not totally free from other proteases. In order to express the rat proCPB in Pichia pastoris, total RNA extracted from SD rat pancreas cells was reversely transcripted to synthesize cDNA, and the proCPB ORF was synthesized by PCR. After digestion with Xho I and EcoR I , the fragment was inserted into pPIC9, and the recombinant plasmid was named as pPIC9-proCPB. By digestion with Sac I , the lined pPIC9-proCPB was transformed into Pichia pastoris strains GS115 with PEG1000 and integrated into their genomes. In the inducement of methanol, recombinant proCPB was successfully expressed in Pichia pastoris, and could be secreted into the supernatant in the culture.
Purity by HPLC
≥ 200 units/mg pro
No chymotrypsin, carboxypeptidase A, or other proteases contaminant.
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to
aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable
within 24 months and no activity lose after 10 times repeated freezing and thawing.
Contact Person: Miss Eland