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Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin

Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin

    • Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin
    • Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin
    • Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin
    • Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin
  • Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: $198/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000 per week
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    Detailed Product Description
    Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
    Source: E. Coli Specific Activity: NLT 200 Units/mg Pro
    Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Sequencing Grade Chymotrypsin
    Impact: Produce Insulin

    Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin

     

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

    MAIN FEATURES

     

    Source Recombinant E. coli
    Appearance White or off white, or yellowish powder
    Specific activity ≥170 units/mg pro.
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 33.8±3.4 kDa

    UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    RECOMMEND USAGE

     

    Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

     

    Recommend Usage

     

    Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

    Carboxypeptidase B Proteomics Grade Producing Recombinant Insulin

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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