|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||100ug|
|Packaging Details:||ice packaging|
|Supply Ability:||1000g per week|
|Product:||Sequencing Grade Carboxypeptidase B||Manufacturer:||YaxinBio|
|Physical Form:||Lyophilized Powder||Specific Activity:||NLT 200 Units/mg Pro|
|Contaminant Activity:||No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant.||Molecular Weight(SDS-PAGE):||33.8±3.4 KDa|
Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥200 units/mg pro.|
|SDS-PAGE||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
Formulation:The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Solubility:It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Purity: Greater than 95% as determined by SDS-PAGE.
Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may
not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below 2-8°C. For long term storage it is recommended to be stored below -20℃.Please prevent freeze-thaw cycles.
Contact Person: Miss Eland