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Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis

Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis

    • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis
    • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis
    • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis
    • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis
    • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis
  • Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Cat. No.: SRT0201
    Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
    Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.

    Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis

     

    Catalog Number:SRT0201

    CAS:9002-07-7
    EC:3.4.21.4

    Storage temperature: 2-8℃

    Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli

    Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin

     

    Description

     

    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    Main Features 

     

    Source

    E. Coli

    Format

    Liquid in 50mM HAc, or fluffy solid.

    Protein concentration

    0.5mg/ml in 50mM HAc

    Purity(RP-HPLC)

    ≥99% by HPLC

    Contaminant activity

    Recombinant porcine trypsin.

    No any other proteases activities contaminant.

     

     

     

     

     

     

     

     

    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    RECOMMEND USAGE

    It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

    Sequencing Grade Recombinant Trypsin For Mass Spectrometric Analysis

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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