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Sequencing Grade Trypsin Digestion of Protein and Polypeptide and Digest Protein

Sequencing Grade Trypsin Digestion of Protein and Polypeptide and Digest Protein

    • Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein
    • Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein
    • Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein
    • Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein
  • Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
    Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
    Purity: One Single Main Band At 24kD By SDS-PAGE RELATED PRODUCT: Recombinant Protein A

    Sequencing Grade Trypsin Digestion of Protein and Polypeptide and Digest Protein

     

    sequencing grade trypsin modified

    Description

     

    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white, or yellowish powder
    Specific activity ≥4500 USP units/mg pro.
    SDS-PAGE Single major band
    Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
    Purity(HPLC)

    ≥95%

     

     

    PRODUCT ADVANTAGE

    Sequence grade recombinant protein: high specificity,good stability.

     

    Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

     

    Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

     

    High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

     

    Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

     

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

     

    Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    Source

    E. Coli

    Format

    Liquid in 50mM HAc, or fluffy solid.

    Protein concentration

    0.5mg/ml in 50mM HAc

    Purity(RP-HPLC)

    ≥99% by HPLC

    Contaminant activity

    Recombinant porcine trypsin.

    No any other proteases activities contaminant.

     

     

     

     

     

     

     

     

    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    Application

     

    Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

     

    Recommend Usage

     

    1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

    2. When using this product, thaw it at room temperature, and mix gently before use.

    3. No activity lost when freeze-thaw 5 cycles.

     

    Storage Instructions

     

    1. The solution should be stored under -70℃, It is stable within 24 months.

    2. Above 95% activity remained after 5 times repeated freezing and thawing.

    3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

    Sequencing Grade Trypsin  Digestion of Protein and Polypeptide and Digest Protein

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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