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Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio

Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio

    • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio
    • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio
    • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio
    • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio
    • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio
  • Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001:2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 198$/100ug
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Cat. No.: 9002-07-7
    Packaging: 100ug 1mg Manufacturer: YaxinBio
    Specific Activity: ≥4500 USP /mg Pro RELATED PRODUCT: Recombinant Carboxypeptidase B

    Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion

     

    sequencing grade trypsin

    Description

     

    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere

    with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin

    is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    RECOMMEND USAGE

    It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

     

    STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

    It is no activity loss after 5 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    Main Features

     

    Source

    E. Coli

    Format

    Liquid in 50mM HAc, or fluffy solid.

    Protein concentration

    0.5mg/ml in 50mM HAc

    Purity(RP-HPLC)

    ≥99% by HPLC

    Contaminant activity

    Recombinant porcine trypsin.

    No any other proteases activities contaminant.

     

     

     

     

     

     

     

     

    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a 

    reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

    Sequencing Grade Recombinant Trypsin for in-gel Protein Digestion by shanghai yaxinbio

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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