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Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics

Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics

    • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics
    • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics
    • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics
    • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics
    • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics
  • Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001:2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 1mg
    Price: 385$/mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Sequencing Grade Trypsin Source: E. Coli
    Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
    Purity: One Single Main Band At 24kD By SDS-PAGE RELATED PRODUCT: Recombinant Trypsin

     

    Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics

     

    Sequencing Grade trypsin

     

    Catalog Number:SRT0202

    CAS:9002-07-7
    EC:3.4.21.4

    Source:Expressed in E. Coli, methylation modified.

     

    Description

     

    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

     

    Application

     

    Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

     

    General description

     

    This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

     

    PRODUCT USAGE

     

    The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

     

    Preparation Note

     

    Stabilizers: Ca2+ in mM concentration range
    Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
    Storage conditions (working solution): -15 to -25°C
    The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

    Sequencing Grade Recombinant trypsin Mass Spectrometry Grade For Proteomics

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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