|Place of Origin:||China|
|Certification:||NSF ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Delivery Time:||5-8 work days|
|Supply Ability:||1000g per week|
|Product:||Recombinant Aprotinin||Cat. No.:||RTI16-S|
|Source:||E. Coli||Purified By:||HPLC|
|Related Product:||Recombinant Porcine Trypsin||Specific Activity:||≥ 3EPU /mg Pro|
Inhibit Plasmin 3 EPU/mg pro Aprotinin from Bovine Lung and Inhibit Kallikrein
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH >10 or <3. Effective concentration is equimolar with protease.
Recombinant Animal Components Free
Recombinant aprotinin is expressed in E. Coli, and purified by HPLC. It contains no animal-derived components. This is a recombinant form of bovine lung aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography.
Unit Definition:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit = 1800 KIU.
|Source||Recombinant E. coli|
|Appearance||Clear, colorless to yellowish liquid|
|Protein Concentrate||≥ 5.0|
|Specific Activity||≥ 3.0EPU /mg protein|
|Absorbance (277nm)||≤ 0.8(3 EPU/ml in water)|
UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.
A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit
Effective concentration is equimolar with protease. The recommend binding is pH＞6.0 and it will dissociate at pH <3.0.
Recombinant aprotinin should be stored under -20°C or below in sealed container.It is stable within 24 months.
Contact Person: Miss Eland