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SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results

SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results

    • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results
    • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results
    • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results
    • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results
    • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results
  • SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RSPA05

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 79$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Source: E.Coli Purified: HPLC
    Bioburden: No Organisms Detected 12% SDS-PAGE: Single Major Band
    Coomassie Stain: 25kD UV Spectrum: E275/250≥1.2, E0.1% At 275nm=0.14-0.20

     

    SPA Binding to IgG Structure of Different Antibodies Protein A and PCR amplification results

     

    Recombinant  Protein  A

    Application

     

    The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

    Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

     

    Product Specification

     

    Test/Method                  

    Specification

    Coomassie Stain

    25kD

    UV Spectrum

    E275/250 ≥1.2,

    E0.1% at 275nm=0.17-0.23 

    UV spectrometry

    No DNA or RNA detected

    Triton Content

    No Triton contaminant

    Format

    Lyophilized

    pH stable range

    pH 1-13

     

     

     

     

     

     

     

     

     

     

     

    STABILITY OF STORAGE AND TRANSPORT

    1. Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.After dissolved with pure water, it should be stored under -20℃.
    2. Stability of transport: The product is stable by blue ice insulation transport.

     

    APPLICATION

     

    The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

     

    SPA Binding to IgG Structure  of Different Antibodies Protein A and PCR amplification results

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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