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Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity

Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity

    • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
    • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
    • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
    • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
  • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity

    Product Details:

    Place of Origin: China, Shanghai
    Brand Name: YAXINBIO
    Certification: NSF ISO 9001:2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging, carton
    Delivery Time: 6 days
    Payment Terms: T/T
    Supply Ability: 1000ku per week
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    Detailed Product Description
    Product: Recombinant Enterokinase Source: E.Coli
    Packaging: 100U, 1KU Or Bulk Fusion Protein Concentration: ≥5 U/μl
    Storage: Store At -20°C After Delivery. RELATED PRODUCT: Sequencing Grade Trypsin
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    enterokinase enzyme

    ,

    recombinant enzymes

    Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
         

    Recombinant Enterokinase 

     

    Catalog Number:REK08

    CAS:9017-74-8
    EC:3.4.21.9

    Storage temperature: -20°C or below

    Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain

     

    Description

     

    YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme

    has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic

    acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or

    tags to get aim protein with native amino acids sequence.

     

    Advantages

     

    1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-

    Asp-Lys (D-D-D-D-K)

    2) No any other contaminated proteases, no non-specific cutting sites.

     

    Recommend Usage Condition:

     

    Cutting condition 25mM Tris-HCl 8.0
    Fusion protein concentration 0.1-1mg/ml (total protein content: 0.5-1.0mg)
    EK content 1-2U
    Temperature 25℃
    Time overnight or 16h-24h for digestion

     

     

     

     

     

     

     

    Biochemphysiol Actions

     

    Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen

    to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer, wherein, the light and the heavy chains

    are linked by two disulfide bridges. Native enterokinase is composed of an 800 amino acid heavy

    chain and a 235 amino acid light chain. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. This cleavage site is incorporated into the FLAG tag.

     

    Physical Form


    supplied as a solution in 20 mM Tris-HCl, 200 mM NaCl, and 50% glycerol

     

    Application

     

    Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic

    activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

    Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential

    activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used

    in a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.

     

    Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity 0

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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