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EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

    • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier
    • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier
    • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier
    • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier
    • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier
  • EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000ku per week
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    Detailed Product Description
    Product: Recombinant Enterokinase Source: E.Coli
    Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. Manufacturer: YaxinBio
    Storage: Store At -20°C After Delivery. Activity: ≥5 U/μl
    Purity(SDS-PAGE): Single Major Band

    EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

     

    Recombinant  Enterokinase

     

    Description

     

    Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.

     

    Common Components Influence The Action of Enterokinase

     

    >200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

    1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

    2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

    3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of Enterokinase in reaction system or extend the reaction time.

     

    Main Features

     

    M.W.

    25,850 Da  

    Specific Activity

    One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 16 to 24 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

    Stability

    Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

     

     

     

     

     

     

     

     

    Product Information

     

    Product

     Activity

    Packaging

    Manufacturer

    Recombinant Enterokinase

    ≥5 u/μl

    100u;500u;1ku; 10ku;20ku;100ku

    YaxinBio

     

     

     

     

     

    Unit Definition

     

    One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

    EC is 3.4.21.9 Recombinant Bovine Enterokinase or Recombinant Enterokinase Supplier

     

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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