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Recombinant Enterokinase E.coli For Removing Unwanted Protein

Recombinant Enterokinase E.coli For Removing Unwanted Protein

    • Recombinant Enterokinase  E.coli For Removing Unwanted Protein
    • Recombinant Enterokinase  E.coli For Removing Unwanted Protein
    • Recombinant Enterokinase  E.coli For Removing Unwanted Protein
    • Recombinant Enterokinase  E.coli For Removing Unwanted Protein
    • Recombinant Enterokinase  E.coli For Removing Unwanted Protein
  • Recombinant Enterokinase  E.coli For Removing Unwanted Protein

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000ku per week
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    Detailed Product Description
    Product: Recombinant Enterokinase Source: E.Coli
    Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. Fusion Protein Concentration: ≥5 U/μl
    Packaging: 100U, 1KU Or Bulk RELATED PRODUCT: Recombinant Human Trypsin

    Recombinant Enterokinase  E.coli For Removing Unwanted Protein 


    Recombinant Enterokinase

     

    Description

    YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

     

    Advantages

    1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

    2) No any other contaminated proteases, no non-specific cutting sites.

     

    Recommend Usage Condition:

    Cutting condition:     25mM Tris-HCl 8.0

    Fusion protein concentration:  0.1-1mg/ml (total protein content: 0.5-1.0mg)

    EK content:      1-2U

    Temperature:   25℃

    Time:    overnight or 16h-24h for digestion.

     

    Common components influence the action of enterokinase

    >200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

    1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

    2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

    3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

     

    Main Features

    M.W.

    25,850 Da  

    Specific Activity

    One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

    Storage Temperature

    -20°C or below.  

    Stability

    Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

     

     

     

     

     

     

     

     

     

     

    Product Information

    Product

     Activity

    Packaging

    Manufacturer

    Recombinant Enterokinase

    ≥5 u/μl

    100u;500u;1ku; 10ku

    YaxinBio


     

     

     

    Recombinant Enterokinase  E.coli For Removing Unwanted Protein

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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