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Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier

Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier

    • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
    • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
    • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
    • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
    • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
  • Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001:2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging, carton
    Delivery Time: 5 days
    Payment Terms: T/T
    Supply Ability: 10,000ku per week
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    Detailed Product Description
    Product: Recombinant Enterokinase Manufacturer: YaxinBio
    Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein. Fusion Protein Concentration: ≥5 U/μl
    Packaging: 100U, 1KU ,10KU RELATED PRODUCT: Recombinant Carboxypeptidase B
    High Light:

    bovine enterokinase

    ,

    recombinant enzymes

    Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier
                

    Recombinant  Enterokinase

    CAS:9017-74-8
    EC:3.4.21.9

    Source: bovine enterokinase, expressed in E. Coli

     

     

    Description

     

    YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme

    has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic

    acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or

    tags to get aim protein with native amino acids sequence.

     

     

    Advantages

     

    1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-

    Asp-Lys (D-D-D-D-K)

    2) No any other contaminated proteases, no non-specific cutting sites.

    Recommend Usage Condition:

    Cutting condition:             given an example: 25mM Tris-HCl 8.0

    Fusion protein concentration:  0.1-1mg/ml (total protein content: 0.5-1.0mg)

    EK content:                   1-2U

    Temperature:                 25℃

    Time:                         overnight or 16h-24h for digestion.

    Common components influence the action of enterokinase >200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

    1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

    2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl,

    <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

    3) If there are one or more components in samples, and cannot be removed, suggest to increase

    the content of EK in reaction system or extend the reaction time.

     

     

    Main Features

     

    Source

    E.Coli

    M.W.

    25,850 Da  

    Specific Activity

    One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 16 to 24 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

    Storage Temperature

    -20°C or below.  

    Stability

    Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

     

     

     

     

     

     

     

     

     

     

     

     

    Product Information

     

    Product

     Activity

    Packaging

    Manufacturer

    Recombinant Enterokinase

    ≥5 u/μl

    100u;500u;1ku; 10ku

    YaxinBio

     

     

     

     

     

    Unit Definition


    One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.

     

    Application


    Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic

    activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.
    Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential

    activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used in

    a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.
    The enzyme from Sigma has been used to compare the specific activity with that of purified, recombinant bovine enterokinase (light chain) overexpressed inEscherichia coli.

    Recombinant Enterokinase, For Removing N-terminal Fusion Protein, Supplier

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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