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Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK

Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK

    • Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK
    • Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK
    • Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK
    • Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK
  • Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000ku per week
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    Detailed Product Description
    Source: E. Coli Time: Overnight Or 16h-24h For Digestion.
    Cat.No: REK08 Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.
    Fusion Protein Concentration: 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) Cutting Condition: Given An Example: 25mM Tris-HCl 8.0

    Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK

     

    Recombinant Bovine Enterokinase

    CAS:9017-74-8
    EC:3.4.21.9

    Source: bovine enterokinase, expressed in E. Coli

     

    Description

     

    YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

     

     

    MAIN FEATURES

     

    Source Recombinant E. coli
    Appearance Clear,colorless to yellowish liquid
    Activity ≥5 u/μl
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 25.8 ±2.6 kDa

    UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to 16 hours to get 95% completion at 25°C in 25 mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

     

     

    Advantages

     

    Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)
    No any other contaminated proteases, no non-specific cutting sites

     

     

     

     

     

    Product Information

     

    Product

     Activity

    Packaging

    Manufacturer

    Recombinant Enterokinase

    ≥5 u/μl

    100u;500u;1ku; 10ku

    YaxinBio

     

     

     

     

    Recombinant Bovine Enterokinase Bovine Enterokinase or DDDDK

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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