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Recombinant Enterokinase and Recombinant Bovine Enterokinase

Recombinant Enterokinase and Recombinant Bovine Enterokinase

    • Recombinant Enterokinase and Recombinant Bovine Enterokinase
    • Recombinant Enterokinase and Recombinant Bovine Enterokinase
    • Recombinant Enterokinase and Recombinant Bovine Enterokinase
    • Recombinant Enterokinase and Recombinant Bovine Enterokinase
  • Recombinant Enterokinase and Recombinant Bovine Enterokinase

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 10000ku per week
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    Detailed Product Description
    Source: E. Coli M.W.: Theoretical MW: 25,850 Da;The Apparent MW On SDS-PAGE: About 27,000 Da
    Storage: Store At -20°C After Delivery. Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.
    Fusion Protein Concentration: 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) Cutting Condition: Given An Example: 25mM Tris-HCl 8.0

    Recombinant Enterokinase and Recombinant Bovine Enterokinase

     

    Recombinant Enterokinase

    Product Overview

     

    Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.

     

    Main Features

     

    M.W.

    25,850 Da  

    Specific Activity

    One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

    Stability

    Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

     

     

     

     

     

     

     

     

    RECOMMEND USAGE CONDITION:

    1) Cutting condition: 25mM Tris-HCl 8.0

        Fusion protein concentration: 0.1-1mg/ml (total protein content: 50-100ug)

        EK content: 0.1-0.2U

       Temperature: 25℃

       Time: overnight or 16h-24h for digestion.

    If digestion under 2-8°C,prolong the digestion time two folds,or more enzyme added to short the digestion time.

    2) 4°C low temperature digestion conditions:

        4°C can effectively digest the substrate, but need to extend the digestion time to 48-64h, or increase the amount of 2-3 times the amount of enzyme.

    3) Enzyme cleavage optimization and amplification

        Optimization: Enzyme cleavage conditions can be optimized, such as buffer pH, fusion protein concentration, EK amount, and digestion time, so that the fusion protein can be digested under stable conditions, SDS-PAGE detection of digestion effect.

        Amplification: Select the optimized reaction conditions, according to the conditions such as the proportion of amplification, SDS-PAGE detection of digestion effect.

    4) Method for removing recombinant enterokinase: The recombinant enterokinase was removed using trypsin inhibitor affinity chromatography (e.g., sigma T0637).

    5) Note: do not recommend cutting at 37°C conditions, there may be non-specific enzyme digestion.

     

     

    COMMON COMPONENTS INFLUENCE THE ACTION OF ENTEROKINASE

    >200mM imidazole or >200mM NaCl or >5% glycerin, the reaction may be effected.The following suggestions are given:

    1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

    2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

    3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

     

    Product Information

     

    Product

     Activity

    Packaging

    Manufacturer

    Recombinant Enterokinase 

    ≥5 u/μl

    100u;500u;1ku;10ku;100ku

    YaxinBio

     

     

     

     

     

    Unit Definition

     

    One unit is defined as the amount of enzyme needed to cleave 500 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

    Recombinant Enterokinase and Recombinant Bovine Enterokinase

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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