|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||1mg|
|Packaging Details:||ice packaging|
|Delivery Time:||7 Days|
|Supply Ability:||1000g per week|
|Product:||Recombinant Human Trypsin||Packaging:||10mg,100mg Or Bulk|
|Source :||E. Coli||Purified by:||HPLC|
|Related Product:||Recombinant Porcine Trypsin||Manufacturer:||YaxinBio|
Stable Recombinant Human Trypsin Enzyme Expressed in E.Coli and Purified
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end
of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited
by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen . Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas
and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes . Mutations in the PRSS1 gene can
cause hereditary pancreatitis
White or White-like lyophilized
≥ 2500 USP u/mg pro.
≥ 95% by HPLC
No chymotrypsin, carboxypeptidase A, and other protease contaminant.
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in
a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is
stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months
and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland