|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Supply Ability:||1000g per week|
|Source:||E. Coli||Cat. No.:||RHT03|
|Manufacturer:||YaxinBio||Contaminant Activity:||No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.|
|Related Product:||Recombinant Porcine Trypsin||Packaging:||10mg,100mg Or Bulk|
stem cell culture
Animal Components Free , Recombinant Human Trypsin for Treating Cell
Source: human trypsin, expressed in E. Coli.
1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.pH7.0-9.0 is the optimum pH. Serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA can inhibite the trypsin enzyme.
2.Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥2500 USP units/mg pro.|
|Protein Content||≥ 90%|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Recombinant human trypsin lyophilized powder should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland