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Animal Components Free , Recombinant Human Trypsin for Treating Cell

Animal Components Free , Recombinant Human Trypsin for Treating Cell

    • Animal Components Free , Recombinant Human Trypsin for Treating Cell
    • Animal Components Free , Recombinant Human Trypsin for Treating Cell
    • Animal Components Free , Recombinant Human Trypsin for Treating Cell
    • Animal Components Free , Recombinant Human Trypsin for Treating Cell
  • Animal Components Free , Recombinant Human Trypsin for Treating Cell

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RHT03

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 89$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Source: E. Coli Cat. No.: RHT03
    Manufacturer: YaxinBio Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.
    Related Product: Recombinant Porcine Trypsin Packaging: 10mg,100mg Or Bulk

    Animal Components Free , Recombinant Human Trypsin for Treating Cell

     

    CAS: 9002-07-7
    EC: 3.4.21.4

    Source: human trypsin, expressed in E. Coli.

     

    Description

     

    1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.pH7.0-9.0 is the optimum pH. Serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA can inhibite the trypsin enzyme.

     

    2.Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

     

    MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white powder
    Specific activity ≥2500 USP units/mg pro.
    Protein Content ≥ 90%
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
    Purity(HPLC) ≥ 95%

    UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    RECOMMEND USAGE

    Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

     

    Storage Instructions

    Recombinant human trypsin lyophilized powder should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Animal Components Free , Recombinant Human Trypsin for Treating Cell

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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