|Place of Origin:||Xuhui, Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||1mg, 10mg, 100mg, 1g; ice packaging, carton|
|Supply Ability:||1000g per week|
|Product:||Recombinant Human Trypsin 2||Packaging:||10mg,100mg Or Bulk|
|Specific Activity:||≥2500 USP U/mg Pro.||Contaminant Activity:||No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.|
|Related Product:||Recombinant Porcine Trypsin||Manufacturer:||YaxinBio|
stem cell culture
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Recombinant Human Trypsin
|1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.|
|2.Recombinant human trypsin is protein expressed in E.coli through a genetically engineered and purified by high pressure liquid chromatography. Enzyme activities such as carboxypeptidase A and chymotrypsin are not contained in the trypsin. No protease inhibitors such as PMSF are contained in the preparation.|
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥2500 USP units/mg pro.|
|Protein Content||≥ 90%|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
Animal origin free:The use of recombinant human trypsin eliminates the risk of virus presence and any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio recombinant human trypsin belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as the human trypsin 2.
Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
|Recombinant human trypsin provides increased specificity and eliminates
contaminating activities found in lower purity enzymes.
|No other contaminating proteases such as chymotrypsin or carboxypeptidase A.|
|Purity is more than 95% by HPLC.|
Contact Person: Miss Eland