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E.coli Recombinant Trypsin Animal Origin Free Trypsin 3800 USP u/mg Pro

E.coli Recombinant Trypsin Animal Origin Free Trypsin 3800 USP u/mg Pro

    • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
    • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
    • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
    • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
    • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
  • E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RPT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 65$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Recombinant Porcine Trypsin Physical Form: White Lyophilized Powder
    Specific Activity: ≥3800 USP U/mg Pro Protein Content: 35% ~ 70%
    Related Product: Sequencing Grade Trypsin

    E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro
     

     

    Description

     

    Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,e.g.EDTA.

     

    Assay Method
    The activity of most Sigma preparations is determined by a continuous rate spectrophotometric assay

    and expressed in BAEE units.

    Unit Definition: One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

     

    Conditions
    Temp = 25 °C, pH = 7.6, A253nm, Light path = 1 cm
    In a 3.2 ml reaction mix, the final concentrations are 63 mM sodium phosphate, 0.23 mM Nα-benzoyl-L-arginine ethyl ester, 0.06 mM hydrochloric acid, and 100 units trypsin.

     

    Unit Conversions

    1 BAEE µM Unit = 200 BAEE Units
    1 TAME µM Unit = 0.27 BAEE µM Units
    1 BAEE µM Unit = 3.64 TAME Units
    1 TAME µM Unit = 55 BAEE A253 Units
    1 BAEE A253 Unit = 0.018 TAME µM Unit
    1 TAME µM Unit = 180 TAME A247 Units
    1 TAME A247 Unit = 0.33 BAEE Units
    1 USP Unit = 3.0 BAEE Units
    1 NF Unit = 1.1 USP Units

     

    Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

    by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

     

    Advantages

    Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

    and other potential adventitious agents found in animal-derived trypsin.

    YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

    Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

     

    Main Features

     

    Source

    E. Coli

    Purified by

    HPLC

    Purity(RP-HPLC)

    NLT 70% β-trysin, NMT 20% α-trypsin

    Contaminant activity

    No chymotrypsin, carboxypeptidase A, and other protease contaminant.

     

     

     

     

     

     

     

    Recommend Usage

     

    Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.

     

    Storage Instructions

     

    Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    E.coli Recombinant Trypsin  Animal Origin Free Trypsin 3800 USP u/mg Pro

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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