|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Delivery Time:||8 days|
|Supply Ability:||1000g per week|
|Manufacturer:||YaxinBio||Packaging:||10mg,100mg,1g Or Bulk|
|Source:||E. Coli||Physical Form:||White Lyophilized Powder|
|Related Product:||Recombinant Human Trypsin|
Edta Trypsin Proteomics Grade Tpck Trypsin Recombinant Trypsin
Enzyme Commission (EC) Number: 22.214.171.124
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(bovine)
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end
of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7.0 - 9.0. The enzyme
is inhibited by serine protease inhibitors, e.g. PMSF, aprotinin, soybean trypsin inhibitor and by
metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no any other contaminating enzyme activities.
No protease inhibitors such as PMSF are contained in the preparation.
Animal origin free:The use of recombinant porcine trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
|Specific activity||≥3800 USP units/mg pro|
|Purity(RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
|Contaminant activity||No chymotrypsin, carboxypeptidase A, and other protease contaminant.|
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute
in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
To prepare 1-10mg/ml recombinant trypsin with 1mM HCl. The ratio to aimed protein is 1:50 to
1:1000 (w/w), the optimum pH is pH7-10.
Storage And Stability
Recommend recombinant trypsin lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Contact Person: Miss Eland