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Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant trypsin

Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant trypsin

    • Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin
    • Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin
    • Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin
    • Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin
  • Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RPT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 65$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Manufacturer: YaxinBio Cat. No.: RPT0201
    Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant. Physical Form: White Lyophilized Powder
    Related Product: Recombinant Human Trypsin Impact: Dissociate Cells
    Source: Expressed In E.coli.

    Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant trypsin
     

    Recombinant Trypsin Solution

     

    Description

    Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

     

    Advantages

     

    Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

    Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

     

    Main Features

     

    Source

    E. Coli

    Purified by

    HPLC

    Specific activity

    ≥3800 USP units/mg pro

    Purity(RP-HPLC)

    NLT 70% β-trysin, NMT 20% α-trypsin

    Contaminant activity

    No chymotrypsin, carboxypeptidase A, and other protease contaminant.

     

     

     

     

     

     

     

    Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    Recommend Usage

     

    Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.

     

    Storage Instructions

     

    Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     

    Cell Dissociation Solution Expressed in E.coli for Dissociate Cells which is recombinant  trypsin

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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